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KMID : 0385520210340030115
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Analytical Science & Technology
2021 Volume.34 No. 3 p.115 ~ p.121
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Method validation of detecting ethanol metabolites (EtG, EtS) in post-mortem spleen
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Kim Soo-MIn
Jo Young-Hoon
Ah Song-Hie
Lee Woo-Jae
Kwon Mi-A
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Abstract
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Ethyl glucuronide (EtG) and ethyl sulfate (EtS), which are ethanol metabolites, are direct indicators of ethanol intake; they have been studied in a variety of biological samples in forensic science. It is necessary to analyze ethanol metabolites to determine whether the ethanol detected in autopsy cases was due to alcohol consumption before death or due to the ethanol produced from post-mortem decay. In general, EtG and EtS are detected in the blood together with ethanol; however, it may be difficult to secure blood depending on the extent of decay. Therefore, the aforementioned method should be replaced by detecting the ethanol metabolites using tissue biological samples. In this study, we determined the optimal experimental conditions for detecting EtG and EtS from spleen samples using Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS).
Herein, the test method was validated, and an analysis method was applied to the actual autopsy cases.
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KEYWORD
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ethanol, metabolite, ethyl glucuronide, ethyl sulfate, spleen
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